| 1A | FHSAA MDE 1A | 11.49 |
| 2A | FHSAA MDE 2A | 11.23 |
| 3A | FHSAA MDE 3A | 11.33 |
| 4A | FHSAA MDE 4A | 11.27 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | West, Jared | Wiregrass Ranch | 11.70 | Berkeley Track and Field Invitational |
| 2 | Queen, Willie | Zephyrhills | 11.59 | Cypress Creek QUAD 2020 |
| 3 | Jackson, Timothy | Zephyrhills | 11.37 | Zephryhills High School Tri Meet |
| 4 | Neal, Maguire | Zephyrhills | 11.20 | Springstead High School Invitational |
| 5 | Jackson, Dontrevius | Bishop McLaughlin | 11.29 | Berkeley Tailgate Track & Field Meet |
| 6 | Otero, Jayden | Land O'Lakes | 11.55 | Berkeley Track and Field Invitational |
| 7 | Ayan, Riddyk | Wiregrass Ranch | 11.66 | Berkeley Track and Field Invitational |
| 8 | Patten, Jayven | Land O'Lakes | 12.01 | George Steinbrenner High School Track and Field Invite |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Hawkins, Cole | Mitchell | 12.69 | Berkeley Track and Field Invitational |
| 2 | Lupu, Davian | Fivay | 12.27 | Fivay Invitational |
| 3 | Ames, Caleb | Land O'Lakes | 12.17 | Berkeley Track and Field Invitational |
| 4 | Ayala, Kennen | Land O'Lakes | 12.06 | Anclote High School Meet One |
| 5 | Silva, Manny | Wiregrass Ranch | 12.12 | Cypress Creek Quad 2021 |
| 6 | Fayiz, Aydin | Wiregrass Ranch | 12.25 | Berkeley Track and Field Invitational |
| 7 | Shaw, Chris | Fivay | 12.46 | Anclote High School Meet One |
| 8 | Lopez, Elijah | Mitchell | 12.73 | East Lake Early Bird Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Fowler, Deyante | Fivay | NT | |
| 2 | Haynes, Leon | Bishop McLaughlin | NT | |
| 3 | Rutledge, Aiken | Mitchell | 13.07 | Berkeley Track and Field Invitational |
| 4 | Burnette, Dayson | Mitchell | 12.99 | East Lake Early Bird Invitational |
| 5 | Glenn, Alijah | Fivay | 13.00 | River Ridge Meet #1 |
| 6 | Sands, Theodore | Zephyrhills | 13.37 | Zephryhills High School Tri Meet |
| 7 | Miller, Jack | Bishop McLaughlin | NT |