| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Fivay | A | Keyshawn | Moodie | Glenn | Shaw | 3:51.02 | Hernando Kiwanis Track and Field Invitational |
| 3 | Land O'Lakes | A | Massie | Mccloud | Pryce | Austin | 3:38.56 | Sunshine Athletic Conference East 2019 |
| 4 | Mitchell | A | Capille | Saintvalle | Hindman | Burnette | DNS | FHSAA 4A Region 2 and FHSAA 3A Region 2 |
| 5 | Zephyrhills | A | Clemens | Neal | Gomez | Cornelius | 3:30.50 | Springstead High School Invitational |
| 6 | Wiregrass Ranch | A | Ayan | Spearin | Jhoda | Medina | 3:45.00 | |
| 7 | Bishop McLaughlin | A | Campos | Gaskin | Vazquez | Rios-Rosales | DNS |