| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Killmeyer, Leanne | Tampa |
NT | |
| 2 | Burrows, Robyn | Saint Leo |
1:02.40 | UAB Green and Gold Invitational |
| 3 | Towers, Sara | Embry-Riddle |
1:01.31 | Embry-Riddle First Chance Meet |
| 4 | Dudley, Morgan | Embry-Riddle |
58.55 | South Carolina Invitational |
| 5 | Harrison, Samiya | Embry-Riddle |
59.00 | |
| 6 | Aguilar, Skyelar | Saint Leo |
1:01.61 | South Carolina Invitational |
| 7 | Wilson, Zahra | Tampa |
1:03.49 | Tampa Track Classic #2 |
| 8 | O'Regan, Noelle | Tampa |
1:04.40 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Jarreau, Tania | Saint Leo |
NT | |
| 2 | Chummar, Nithya | Embry-Riddle |
1:06.17 | ERAU First Chance Last Chance |
| 3 | Sisson, Macie | Tampa |
1:04.60 | Tampa Track Classic #2 |
| 4 | Clode, Georgia | Saint Leo |
1:04.47 | Alan Connie Shamrock Invite |
| 5 | Boger, Vyctoria | Saint Leo |
1:04.56 | UAB Green and Gold Invitational |
| 6 | Taylor, Jasmine | Edward Waters |
1:05.15 | SSU Tiger Orange and Blue |
| 7 | Sclater, Sabrina | Edward Waters |
1:06.86 | Savannah State Eyeopener |