| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Edward Waters |
A | Sclater | Taylor | Taylor | Brown | 4:23.91 | Savannah State EyeOpener |
| 2 | Florida Southern |
A | Procopio | Flemming | Guerra | Barth | DNS | ERAU First Chance Last Chance |
| 3 | Tampa |
A | Christian | Riley | Lockridge | Wilson | 4:09.50 | Tampa Track Classic |
| 4 | Embry-Riddle |
B | Friday | McBride | Miller | Thompson | DNS | USC Open Indoor |
| 5 | Embry-Riddle |
A | Richardson | Dudley | Beckwith | Harrison | 3:55.02 | USC Open Indoor |
| 6 | Tampa |
B | Sisson | Killmeyer | O'Regan | Sullivan | DNS | Tampa Track Classic |
| 7 | Saint Leo |
A | Boger | Burrows | Cave | Aguilar | DNS | South Florida Collegeiate Invitational |
| 8 | Lynn |
A | Philips | Madden | Rodriguez | Ramirez | DNS |