| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Israel, Samuel | Edward Waters |
23.40 | UNF Invitational |
| 2 | Delicieux, Cassandrew | Embry-Riddle |
22.70 | |
| 3 | Sydnor, Jhaden | Embry-Riddle |
22.50 | |
| 4 | Joseph, Sandro | Warner |
21.63 | South Florida Collegeiate Invitational |
| 5 | Neely, Meshawn | Warner |
21.91 | USF Bulls Invitational |
| 6 | Thomas, Darrell | Embry-Riddle |
22.68 | Carolina Challenge |
| 7 | Guinn, Connor | Embry-Riddle |
22.90 | |
| 8 | Kafka, Brendan | Tampa |
23.88 | Tampa Track Classic |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | Swad, Jacob | Tampa |
25.09 | ERAU First Chance Last Chance |
| 2 | McCants, Jaquan | Edward Waters |
24.54 | Savannah State Eyeopener |
| 3 | Gentry, Maurice | Tampa |
24.23 | Tampa Track Classic #2 |
| 4 | James, Austin | Tampa |
23.99 | South Florida Collegeiate Invitational |
| 5 | Keene, J'Von | Edward Waters |
24.01 | Savannah State Eyeopener |
| 6 | Cathey, Hayden | Florida College | 24.24 | Embry-Riddle Bahamas House First Chance Last Chance Meet |
| 7 | Sands, Andrew | Saint Leo |
24.57 | Tampa Track Classic #2 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | Shinde, Vineet | Saint Leo |
NT | |
| 4 | Oliver, Dylan | Florida Southern |
25.23 | Tampa Track Classic |
| 5 | Brown, Tramaine | Warner |
25.41 | ERAU First Chance Last Chance |