| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | LONG, Hunter | Mulberry | NT | |
| 4 | SMOTHERS, Alfred | Winter Haven | NT | |
| 5 | GANT , Tayler | Lakeland | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | CLOUTHIER, Benjamin | Mount Dora | NT | |
| 2 | LORISTON, Ethan | Haines City | NT | |
| 3 | HARRIS, Dwayne | Haines City | NT | |
| 4 | CANINO, Anthony | Tampa Bay HEAT | NT | |
| 5 | COLLINS, ReeMari | Lakeland Christian | NT | |
| 6 | DIXON III, James | Mount Dora | NT | |
| 7 | PETERS , Braeden | McKeel | NT | |
| 8 | KELLUM, Reggie | Lakeland | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | WILSON, Derrick | Winter Haven | 27.89 | KHS Open |
| 2 | VELOSO, Joshua | McKeel | 26.10 | Lakeland City Meet 2020 |
| 3 | MORRIS, Clifton | Lake Region | 25.52 | George Jenkins Invite 2021 |
| 4 | SCOTT, Andrew | Mount Dora | 25.25 | St. Cloud Track Invitational |
| 5 | HARRISON, Shadarian | Kathleen | 25.46 | St. Cloud Track Invitational |
| 6 | MILLS, Brenden | George Steinbrenner | 25.59 | 48th RAM Invite 2021 |
| 7 | COLLINS, Ryan | Tampa Bay HEAT | 27.80 | Wildwood High School Invitational |
| 8 | CURRY, Jamel | Mulberry | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | QUINTANA, Lucas | George Steinbrenner | 24.86 | Kincaid Classic 2021 |
| 2 | ARIAS ROSALES, Andrews | Celebration | 24.76 | Satellite High Reggie Johnson Invite 2021 |
| 3 | SMITH, Breylin | Lakeland Christian | 24.54 | St. Cloud Track Invitational |
| 4 | WELCH, Gary | Plant City | 24.50 | |
| 5 | CAPRON, John | George Jenkins | 24.50 | |
| 6 | HART, Valentino | Tampa Prep | 24.72 | Calvary Christian Warrior Invite 2021 |
| 7 | HILL, Bryan | Winter Haven | 24.86 | George Jenkins Invite 2021 |
| 8 | GREEN, Calvert | Mulberry | 25.10 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | JACKSON, Quavantez | Kathleen | 23.97 | George Jenkins Invite 2021 |
| 2 | RUCKER, D'Marius | Lakeland | 23.84 | St. Cloud Track Invitational |
| 3 | WILLIAMS, Idris | Lakeland Christian | 23.34 | George Jenkins Invitational Track Meet 2020 |
| 4 | STITH, Justin | Dr. Phillips | 23.27 | St. Cloud Track Invitational |
| 5 | DOAN, Joshua | George Steinbrenner | 23.33 | East Lake Early Bird Invitational |
| 6 | HUDSON, Jeremiah | George Jenkins | 23.37 | Brian Jaeger Elite Classic 2020 |
| 7 | METCALF, Kameryn | Dr. Phillips | 23.92 | George Jenkins Invite 2021 |
| 8 | BURGESS, Devin | George Jenkins | 24.00 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | VALENTINE, Ja'Darian | Haines City | 22.97 | |
| 2 | JOLLY, Ian | Plant City | 22.90 | |
| 3 | ALLS, Anthony | Auburndale | 22.50 | |
| 4 | PIERRE, Emmanuel | Dr. Phillips | 21.98 | 2019 FHSAA State Track & Field Championships |
| 5 | DONALDSON, Christopher | Auburndale | 22.12 | George Jenkins Invitational Track Meet 2020 |
| 6 | JOHNSON, Keyshawn | Kathleen | 22.83 | George Jenkins Invite 2021 |
| 7 | BENNETT, Montrael | Plant City | 22.90 | |
| 8 | POLLARD, Jerry | Auburndale | 23.23 | George Jenkins Invite 2021 |