| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MCGILL, Joesph | Lakeland | 2:34.25 | George Jenkins Invite 2021 |
| 2 | DENTON, Jadin | Lakeland Christian | 2:35.99 | George Jenkins Invite 2021 |
| 3 | CONCEPCION , Ricardo | Lakeland | 2:38.46 | George Jenkins Invite 2021 |
| 4 | OSBORNE, Luke | Tampa Bay HEAT | 2:53.09 | IMG Academy Invitational Fueled By Gatorade |
| 5 | BAILEY, Aiden | Tampa Bay HEAT | 3:00.08 | IMG Academy Invitational Fueled By Gatorade |
| 6 | BLAISE, Wolf | Winter Haven | 3:12.38 | Berkeley Track and Field Invitational |
| 7 | AMOAH-MENSAH, Afrifa | Mount Dora | NT | |
| 8 | DAMRON, Walker | Tampa Bay HEAT | NT | |
| 9 | LABOY-CRUZ, Abdel | Mulberry | NT | |
| 10 | MIRANDA, Manuel | Winter Haven | NT | |
| 11 | PHILPOT, Cooper | Lakeland Christian | NT | |
| 12 | WELLS, Parker | Mulberry | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | WASENDORF, Erik | Dr. Phillips | 2:16.02 | |
| 2 | REED, Essmal | Kathleen | 2:17.30 | St. Cloud Track Invitational |
| 3 | ABANSES, Javier | George Steinbrenner | 2:18.00 | |
| 4 | WENZEL, Maxwell | Tampa Prep | 2:18.86 | Calvary Christian Warrior Invite 2021 |
| 5 | TAO, Brucelee | Auburndale | 2:19.75 | St. Cloud Track Invitational |
| 6 | WHITE, Connor | Tampa Prep | 2:19.75 | Berkeley Track and Field Invitational |
| 7 | JONSSON, Jackson | McKeel | 2:20.23 | KHS Open |
| 8 | VASQUEZ-JUAREZ, Christian | Winter Haven | 2:21.34 | George Jenkins Invite 2021 |
| 9 | JONES, Kalid | Lake Region | 2:21.75 | George Jenkins Invitational Track Meet 2020 |
| 10 | COOK, Connor | McKeel | 2:23.83 | George Jenkins Invitational Track Meet 2020 |
| 11 | VELEZ, Sebastian | Celebration | 2:25.00 | |
| 12 | WRIGHT, Omario | Auburndale | 2:28.33 | Kowboy Developmental Meet |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MCCONNELL, Joel | Dr. Phillips | 2:05.02 | Poinciana Relays |
| 2 | BENDROSS, Gavin | George Jenkins | 2:05.44 | Brian Jaeger Elite Classic 2020 |
| 3 | RUPE, Cameron | George Jenkins | 2:07.00 | |
| 4 | RUPE, Jordan | George Jenkins | 2:07.00 | |
| 5 | WILLS, Erikson | McKeel | 2:07.87 | KHS Open |
| 6 | SIMPSON, Jaden | George Steinbrenner | 2:07.88 | George Steinbrenner Tri Meet #1 |
| 7 | COONS, Avery | Plant City | 2:08.00 | |
| 8 | MCBRIDE, Frank | George Steinbrenner | 2:08.54 | East Lake Early Bird Invitational |
| 9 | EIKEN, Max | Celebration | 2:09.04 | Kowboy Developmental Meet 1 |
| 10 | LEE, Sun Woo | Lakeland Christian | 2:09.37 | FHSAA 1A District 10 |
| 11 | RIVERA, Dylan | Mount Dora | 2:10.54 | St. Cloud Track Invitational |
| 12 | TOUSSAINT, Benjie | Lake Region | 2:10.86 | George Jenkins Invitational Track Meet 2020 |
| 13 | CHO-SAM, Zackary | Mount Dora | 2:12.00 | |
| 14 | AUGUSTIN, Ritchy | Dr. Phillips | 2:13.02 | Poinciana Relays |