| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Dr. Phillips | A | 10:00.39 | Hand Time |
| 2 | George Jenkins | A | 10:20.00 | |
| 3 | Mount Dora | A | 10:28.70 | Storm Preview |
| 4 | McKeel | A | 10:36.00 | |
| 5 | Celebration | A | 10:50.00 | |
| 6 | Tampa Prep | A | 10:52.77 | 2019 FHSAA State Track & Field Championships |
| 7 | Plant City | A | DNS | Hillsborough County Championship |
| 8 | Haines City | A | 11:24.42 | |
| 9 | Lakeland | A | 11:54.49 | St. Cloud Track Invitational |
| 10 | Winter Haven | A | DNS | Poinciana Relays |
| 11 | Lake Region | A | NT |