| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | CHARLES, Jenashia | Haines City | NT | |
| 2 | COLEMAN, Equality | Haines City | NT | |
| 3 | CRUZ, Fabiola | Haines City | NT | |
| 4 | HORNE, Hannah | Dr. Phillips | NT | |
| 5 | THORNTON, Makenzie | McKeel | NT | |
| 6 | CRAFT, Raegan | McKeel | NT | |
| 7 | DAWSON, Alexis | Mount Dora | NT | |
| 8 | CASON, JaMi'ya | Winter Haven | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | DIYORIO, Kyleigh | McKeel | NT | |
| 2 | ROSEL, Eleanora | Mount Dora | NT | |
| 3 | SAXON, Lara | Tampa Bay HEAT | 1:32.96 | Wildwood High School Invitational |
| 4 | BORBOR, Tomasue | Winter Haven | 1:17.53 | Brian Jaeger Elite Classic 2020 |
| 5 | NORTHERN, Saniya | Lake Region | 1:21.01 | George Jenkins Invite 2021 |
| 6 | HUNTER, Claire | Tampa Prep | NT | |
| 7 | HADDAD, Rebekah | Tampa Bay HEAT | NT | |
| 8 | TAYLOR, Ayonna | Mount Dora | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | NIELSEN, Cheylie | Lakeland | 1:12.22 | |
| 2 | DURRANCE, Lacey | George Steinbrenner | 1:11.90 | Kincaid Classic 2021 |
| 3 | POE, Eliza | Tampa Bay HEAT | 1:11.06 | Shorecrest Chargers Invitational |
| 4 | OLIVER, Hefina | Celebration | 1:10.88 | IMG Academy Invitational Fueled By Gatorade |
| 5 | ALLEN, Rebecca | George Steinbrenner | 1:10.90 | |
| 6 | MOON, Ava | George Steinbrenner | 1:11.70 | |
| 7 | ROMERO , Karen | Lakeland | 1:12.00 | |
| 8 | SHEPHERD, LaRhiyyah | Auburndale | 1:16.91 | Kowboy Developmental Meet 1 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | DHILLON, Eran | Celebration | 1:10.03 | IMG Academy Invitational Fueled By Gatorade |
| 2 | CAPELLA, Gabrielle | Tampa Prep | 1:08.92 | Berkeley Track and Field Invitational |
| 3 | QUEVEDO, Alex | Lakeland | 1:08.00 | |
| 4 | JAMERSON, Kenya | Winter Haven | 1:06.27 | Brian Jaeger Elite Classic 2020 |
| 5 | FRETT, Izariah | Dr. Phillips | 1:06.86 | Seminole Invitational at the NTC 2020 |
| 6 | SERRANO, Xenia | George Jenkins | 1:08.00 | |
| 7 | ESPINA-LUKE, Madeline | Mulberry | 1:09.00 | |
| 8 | PARRISH, Jayda | Auburndale | 1:10.81 | Kowboy Developmental Meet 1 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | CRISPIN, Juliette | Plant City | 1:06.00 | |
| 2 | KELLY, Jo'Kierrea | Kathleen | 1:03.99 | 1:03.99 Polk County Championship 2019 |
| 3 | HART, Paris | Kathleen | 1:02.34 | |
| 4 | TILLMAN, Saniyah | Auburndale | 56.65 | 56.65 Berkeley Track and Field Invitational |
| 5 | SAFFORD, Lourdes | Dr. Phillips | 1:01.80 | Larry Kelly Invitational |
| 6 | WRIGHT, Jalayla | George Jenkins | 1:03.00 | |
| 7 | FRAZIER, Alivia | Tampa Prep | 1:06.00 | |
| 8 | BROWN, Lliah | Lake Region | 1:06.26 | George Jenkins Invite 2021 |