| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | MACDONALD, Henry | Lakeland Christian | NT | |
| 4 | THOMPSON, Trey | Lakeland | NT | |
| 5 | DIXON III, James | Mount Dora | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | PALMER, Brody | McKeel | NT | |
| 2 | WILLIAMS, Malachi | Lakeland Christian | NT | |
| 3 | LEVANCE, Justin | Lake Region | 59.90 | George Jenkins Invitational Track Meet 2020 |
| 4 | ANSELL, Kole | George Steinbrenner | 50.71 | Kincaid Classic 2021 |
| 5 | MORRIS, Clifton | Lake Region | 51.34 | George Jenkins Invite 2021 |
| 6 | HOLLAND , Stephen | McKeel | NT | |
| 7 | AYALA, Jayden | Dr. Phillips | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | JOHNSON, Don'Ares | Lakeland | 48.22 | George Jenkins Invite 2021 |
| 2 | MANNO, Christian | George Steinbrenner | 47.16 | 48th RAM Invite 2021 |
| 3 | BANCEY, Davy | Dr. Phillips | 46.32 | George Jenkins Invite 2021 |
| 4 | GADSON, Jaylen | Plant City | 45.52 | George Jenkins Invitational Track Meet 2020 |
| 5 | LITTLES, Tavares | Plant City | 45.82 | Newsome Tri Meet 1 |
| 6 | WASHINGTON, Syhion | Dr. Phillips | 46.75 | St. Cloud Invitational 2020 |
| 7 | ALLEN, Jacob | Lakeland Christian | 47.16 | Cypress Creek Invitational 2020 |
| 8 | LEVERETT , Jadan | Lake Region | 49.87 | George Jenkins Invite 2021 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | RESTREPO, Cayden | George Steinbrenner | 44.75 | 48th RAM Invite 2021 |
| 2 | LUXAMMA, Michael | Mount Dora | 43.70 | Brian Jaeger Elite Classic 2020 |
| 3 | RUNCIE, Khristian | George Jenkins | 42.81 | George Jenkins Invite 2021 |
| 4 | JEAN, Marquis | George Jenkins | 40.92 | Bob English Relays 2020 |
| 5 | BOWERS, Nathan | Haines City | 41.36 | George Jenkins Invite 2021 |
| 6 | ELLIS, La'Quan | George Jenkins | 42.86 | George Jenkins Invite 2021 |
| 7 | APPOLON, Jameson | Haines City | 44.10 | |
| 8 | ESTRADA , Franeudi | Lakeland | 45.44 | George Jenkins Invite 2021 |