| PL | JD | Team | Squad | Time | HPL |
|---|---|---|---|---|---|
| 1 | Seminole (Sanford) | A | 42.02 | 1 (1) | |
| 2 | Mainland | A | 43.77 | 1 (2) | |
| 3 | DeLand | A | 43.88 | 1 (3) | |
| 4 | Olympia | A | 44.10 | 1 (4) | |
| 5 | Forest | A | 44.26 | 1 (5) | |
| 6 | Spruce Creek | A | 45.29 | 1 (6) | |
| 7 | Seabreeze | A | 45.40 | 1 (7) | |
| 8 | University (OC) | A | 45.56 | 2 (1) | |
| 9 | Father Lopez | A | 46.36 | 2 (2) | |
| 10 | Menendez | A | 47.20 | 1 (8) | |
| 11 | Lake Mary | A | 47.27 | 2 (3) | |
| 12 | Matanzas | A | 47.55 | 2 (4) | |
| 13 | Pine Ridge | A | 51.20 | 2 (5) |