| PL | JD | Team | Squad | Time | HPL |
|---|---|---|---|---|---|
| 1 | Seminole (Sanford) | A | 46.99 | 1 (1) | |
| 2 | Spruce Creek | A | 47.22 | 1 (2) | |
| 3 | Seabreeze | A | 47.50 | 1 (3) | |
| 4 | Mainland | A | 47.74 | 1 (4) | |
| 5 | Lake Mary | A | 47.77 | 2 (1) | |
| 6 | University (OC) | A | 48.94 | 1 (5) | |
| 7 | Menendez | A | 49.96 | 2 (2) | |
| 8 | Olympia | A | 51.00 | 1 (6) | |
| 9 | DeLand | A | 54.18 | 1 (7) | |
| 10 | Father Lopez | A | 54.29 | 2 (3) |