| PL | JD | Team | Squad | Time | HPL | Note |
|---|---|---|---|---|---|---|
| 1 | Lake Mary | A | 50.63 | 2 (1) | ||
| 2 | Spruce Creek | A | 53.21 | 1 (1) | ||
| 3 | Forest | A | 54.83 | 1 (2) | ||
| 4 | Seabreeze | A | 56.25 | 1 (3) | ||
| 5 | Olympia | A | 56.69 | 1 (4) | ||
| 6 | Matanzas | A | 57.92 | 2 (2) | ||
| 7 | Mainland | A | 58.49 | 1 (5) | ||
| Father Lopez | A | DQ | out of lane | |||
| Menendez | A | DQ | Zone 2 Violation | |||
| University (OC) | A | DNS |