| PL | JD | Team | Squad | Time | HPL | Note |
|---|---|---|---|---|---|---|
| 1 | Seminole (Sanford) | A | 49.66 | 1 (1) | ||
| 2 | Forest | A | 52.10 | 1 (2) | ||
| 3 | Spruce Creek | A | 52.31 | 1 (3) | ||
| 4 | Menendez | A | 52.86 | 1 (4) | ||
| 5 | DeLand | A | 53.95 | 1 (5) | ||
| 6 | Matanzas | A | 54.50 | 2 (1) | ||
| 7 | Olympia | A | 54.84 | 1 (6) | ||
| 8 | Mainland | A | 55.05 | 1 (7) | ||
| 9 | Lake Mary | A | 56.35 | 2 (2) | 56.348 | |
| 10 | New Smyrna Beach | A | 56.35 | 2 (3) | 56.350 | |
| 11 | University (OC) | A | 56.73 | 2 (4) | ||
| 12 | Pine Ridge | A | 57.54 | 1 (8) | ||
| 13 | Father Lopez | A | 57.87 | 2 (5) |