| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | DeLand | A | 3:45.04 | Deltona Home Meet #1 |
| 2 | University (OC) | A | 3:41.10 | Flagler Volusia Freshman Sophomore Meet |
| 3 | Spruce Creek | A | 3:34.05 | East Coast Classic |
| 4 | Mainland | A | DNS | East Coast Classic |
| 5 | Olympia | A | 3:28.82 | 3:28.82 Metro West Championship 2019 |
| 6 | Forest | A | 3:39.80 | East Coast Classic |
| 7 | Seminole (Sanford) | A | 3:42.00 | |
| 8 | Lake Mary | A | 3:50.00 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Father Lopez | A | 4:20.00 | |
| 4 | Matanzas | A | 3:50.00 | |
| 5 | Seabreeze | A | DQ | Flagler Volusia Freshman Sophomore Meet |
| 6 | Menendez | A | NT |