| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | WHITE, Kanaria | SO | Bartow | NT | |
| 4 | HELMS, Jaymyra | SO | Ridge Community | NT | |
| 5 | HENNINK, Ariel | FR | Vanguard (LW) | NT | |
| 6 | KAZMIER, Hannah | FR | Bartow | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MACDONALD, Ellisa | 6 | Winter Haven Christian School | NT | |
| 2 | FLORES, Kayra | FR | Winter Haven | NT | |
| 3 | BROOKS, Mickayla | FR | Lakeland | 39.49 | The Bob English 2021 |
| 4 | WRIGHT, Zenaria | 7 | Fort Meade | 31.86 | |
| 5 | DUNLAP, Janiah | JR | Lake Region | 31.90 | George Jenkins Invite 2021 |
| 6 | GARVIN, Trinity | SO | Haines City | NT | |
| 7 | CARR, Xzaria | SO | Vanguard (LW) | NT | |
| 8 | WOODHEAD , Christine | SR | Lake Region | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ROMERO , Karen | SO | Lakeland | 31.50 | |
| 2 | GLASGOW, Tasia | JR | Ridge Community | 30.47 | The Ridge Track & Field Invitational |
| 3 | LASTER, Symauri | SO | Lake Wales | 28.97 | |
| 4 | GLASGOW, Shanique | SO | Ridge Community | 28.20 | |
| 5 | DUCLOS, Vanessa | JR | Winter Haven | 28.63 | Polk County Championship 2019 |
| 6 | HAZLETT, Gabrielle | SO | Winter Haven | 29.94 | George Jenkins Invite 2021 |
| 7 | DAVIDSON, Arianna | SO | Fort Meade | 31.41 | |
| 8 | INGRAHAM, Alyssa | Frostproof | 31.76 | Tohopekaliga Invitational 1 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TAYLOR, Kiera | JR | Haines City | 27.64 | The Bob English 2021 |
| 2 | BONDS, Areeyanna | FR | Lake Wales | 26.97 | IMG Academy Invitational Fueled By Gatorade |
| 3 | JOHNSON, Donaria | FR | Lakeland | 26.60 | |
| 4 | WHITEHURST, Azaria | FR | Fort Meade | 25.44 | The Ridge Track & Field Invitational |
| 5 | LOPEZ, Maddie | SO | Lakeland Christian | 26.38 | The Bob English 2021 |
| 6 | JENKINS, Tyla | SO | Lake Wales | 26.70 | Lake Mary Invitational 2021 |
| 7 | FUERY, Trinity | JR | Bartow | 27.41 | The Ridge Track & Field Invitational |
| 8 | DEYANT, Saramide | JR | Haines City | 27.95 | George Jenkins Invite 2021 |