| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | WORKMAN, Sarah | 7 | Lakeland Christian | NT | |
| 4 | VALENZUELA, Ruby | JR | Ridge Community | NT | |
| 5 | WHITE, Kanaria | SO | Bartow | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HUTCHINSON, Ja'Narie | FR | Bartow | NT | |
| 2 | JACKETT, Christie | SO | Lake Wales | 1:24.29 | |
| 3 | WRIGHT, Zenaria | 7 | Fort Meade | 1:21.09 | |
| 4 | DAVIDSON, Arianna | SO | Fort Meade | 1:18.13 | |
| 5 | NIELSEN, Cheylie | JR | Lakeland | 1:20.20 | |
| 6 | TUCKER, Mariah | FR | Winter Haven | 1:22.10 | George Jenkins Invite 2021 |
| 7 | WALLS, Amelia | 7 | Winter Haven Christian School | 1:30.13 | St. Cloud Track Invitational |
| 8 | STABLER, Lydia | 7 | Lakeland Christian | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | INGRAHAM, Alyssa | Frostproof | 1:16.32 | Toho Invitational 2020 | |
| 2 | COLEMAN, Equality | JR | Haines City | 1:14.91 | The Bob English 2021 |
| 3 | WHITEHURST, Azaria | FR | Fort Meade | 1:12.67 | The Ridge Track & Field Invitational |
| 4 | CHARLES, Jenashia | JR | Haines City | 1:11.04 | The Bob English 2021 |
| 5 | NORTHERN, Saniya | FR | Lake Region | 1:12.37 | The Bob English 2021 |
| 6 | QUEVEDO, Alex | JR | Lakeland | 1:14.00 | |
| 7 | POWELL, Samantha | JR | Ridge Community | 1:15.00 | |
| 8 | KAZMIER, Hannah | FR | Bartow | 1:16.39 | George Jenkins Invite 2021 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GLASGOW, Tasia | JR | Ridge Community | 1:10.00 | |
| 2 | JAMERSON, Kenya | SR | Winter Haven | 1:06.27 | Brian Jaeger Elite Classic 2020 |
| 3 | LOPEZ, Maddie | SO | Lakeland Christian | 1:04.31 | Lakeland City Meet 2020 |
| 4 | JENKINS, Tyla | SO | Lake Wales | 59.00 | |
| 5 | BONDS, Areeyanna | FR | Lake Wales | 1:00.00 | |
| 6 | BROWN, Lliah | JR | Lake Region | 1:06.26 | George Jenkins Invite 2021 |
| 7 | PALMATIR, Chelsie | 7 | Frostproof | 1:08.67 | CSN Invitational Powered by UnderArmour |
| 8 | CRUZ, Fabiola | SR | Haines City | 1:10.43 | The Bob English 2021 |