| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JOHNSON, Cameron | JR | Fort Meade | NT | |
| 2 | HALL, William | SR | Lakeland | NT | |
| 3 | MCKINLEY, Dre'Vies | JR | Lakeland | NT | |
| 4 | MORRIS, Clifton | SO | Lake Region | NT | |
| 5 | FULLER, Allen | SO | Vanguard (LW) | NT | |
| 6 | CHARLOTTE, Christopher | FR | Haines City | NT | |
| 7 | DAVIS, Daithan | SR | Bartow | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PEREZ, Gian | SR | Ridge Community | NT | |
| 2 | COX, Grandin | SO | Lakeland Christian | NT | |
| 3 | KELLY, De'Qune | JR | Lakeland Christian | NT | |
| 4 | GALVA, Darrel | FR | Bartow | NT | |
| 5 | SCREEN, Isaiah | SR | Fort Meade | NT | |
| 6 | BRADFORD, Dakota | FR | Bartow | NT | |
| 7 | STEWART, Jeremiah | JR | Lakeland Christian | NT | |
| 8 | SMITH, Xzavia | JR | Fort Meade | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | READY, Chance | Frostproof | 1:26.54 | Tohopekaliga Invitational 1 | |
| 2 | SELENT, Kolson | 7 | Winter Haven Christian School | 1:06.87 | St. Cloud Track Invitational |
| 3 | PATTERSON, Russell | SO | Lakeland | 1:04.27 | George Jenkins Invite 2021 |
| 4 | WARREN, Edrin | Winter Haven | 1:02.65 | Berkeley Track and Field Invitational | |
| 5 | LIPPETT, Jaden | FR | Winter Haven | 1:04.16 | George Jenkins Invite 2021 |
| 6 | HARRIS, Dwayne | SR | Haines City | 1:05.49 | The Bob English 2021 |
| 7 | PRILLHART, CJ | FR | Winter Haven Christian School | 1:08.59 | St. Cloud Track Invitational |
| 8 | VIRUET, Adrian | SO | Ridge Community | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CRUZ, Xavier | SO | Ridge Community | 1:00.92 | George Jenkins Invite 2021 |
| 2 | LANGS, Robert | FR | Lake Wales | 58.18 | |
| 3 | FORSYETH, Keno | JR | Winter Haven | 56.79 | George Jenkins Invite 2021 |
| 4 | KNIGHTEN , Deandre | SR | Frostproof | 49.14 | CSN Invitational Powered by UnderArmour |
| 5 | TELLIS, Ladarius | SR | Lake Wales | 50.00 | |
| 6 | BAEZ, Daniel | SR | Haines City | 58.05 | George Jenkins Invite 2021 |
| 7 | MOORE, Chevaris | JR | Lake Wales | 58.40 | George Jenkins Invitational Track Meet 2020 |
| 8 | GAMMAGE, Eddie | Frostproof | 1:02.20 | Tohopekaliga Invitational 1 |