| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LIPPETT, Jaden | FR | Winter Haven | NT | |
| 2 | DABREO, Jaidon | SO | Vanguard (LW) | NT | |
| 3 | DAVIS, Dami | SO | Vanguard (LW) | NT | |
| 4 | SCREEN, Isaiah | SR | Fort Meade | NT | |
| 5 | O'NEAL, Killian | 8 | Lakeland Christian | NT | |
| 6 | DAVIS, Darriel | FR | Bartow | NT | |
| 7 | RUTLEDGE, Jeremiah | SO | Bartow | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | VIRUET, Adrian | SO | Ridge Community | NT | |
| 2 | PRILLHART, CJ | FR | Winter Haven Christian School | 28.90 | St. Cloud Track Invitational |
| 3 | JOHN, Randolph | JR | Bartow | 27.48 | Auburndale Bloodhounds Preseason Track and Field Classic |
| 4 | TENIDO, Ryle | FR | Frostproof | 25.20 | CSN Invitational Powered by UnderArmour |
| 5 | JOHNSON, De'Marea | SR | Fort Meade | 25.79 | |
| 6 | SELENT, Kolson | 7 | Winter Haven Christian School | 28.60 | Tohopekaliga Invitational 1 |
| 7 | KELLY, De'Qune | JR | Lakeland Christian | NT | |
| 8 | WALTER, Charles | SR | Ridge Community | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LEVERETT , Jadan | SR | Lake Region | 24.90 | George Jenkins Invite 2021 |
| 2 | HILL, Bryan | JR | Winter Haven | 24.43 | The Bob English 2021 |
| 3 | HARRIS, Dwayne | SR | Haines City | 24.24 | The Bob English 2021 |
| 4 | HARRIS, Corey | SO | Lake Wales | 23.82 | Lake Mary Invitational 2021 |
| 5 | LORISTON, Ethan | JR | Haines City | 24.20 | The Bob English 2021 |
| 6 | RILEY, Robert | SO | Lakeland | 24.39 | St. Cloud Track Invitational |
| 7 | HARDY, Devin | JR | Lake Wales | 24.50 | |
| 8 | SMOTHERS, Alfred | FR | Winter Haven | 25.03 | The Bob English 2021 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | COLE, Jon | JR | Fort Meade | 23.53 | Polk County Championship 2019 |
| 2 | WILLIAMS, Idris | JR | Lakeland Christian | 23.10 | The Bob English 2021 |
| 3 | VALENTINE, Ja'Darian | SR | Haines City | 22.97 | |
| 4 | KNIGHTEN , Deandre | SR | Frostproof | 22.35 | The Ridge Track & Field Invitational |
| 5 | GANT , Tayler | SR | Lakeland | 22.45 | The Bob English 2021 |
| 6 | WARD, Jakari | SO | Ridge Community | 22.97 | George Jenkins Invite 2021 |
| 7 | RUCKER, D'Marius | FR | Lakeland | 23.39 | The Bob English 2021 |
| 8 | MICKEL, Laterrian | SO | Lake Wales | 23.75 |