| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | JOHNSON , Markell | FR | Lakeland | NT | |
| 4 | COLLINS, ReeMari | SO | Lakeland Christian | 1:02.76 | St. Cloud Track Invitational |
| 5 | HARRIS, Antonio | FR | Victory Christian (LL) | NT | |
| 6 | JONES, Larry | SO | Lakeland | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CHARLOTTE, Christopher | FR | Haines City | 1:00.91 | The Ridge Championship 2021 |
| 2 | ZACEK, Sebastian | McKeel | 59.30 | George Jenkins Tri Meet | |
| 3 | WILSON, Derrick | SR | Winter Haven | 58.71 | Brian Jaeger Elite Classic 2020 |
| 4 | CHESHIRE, Joseph | JR | George Jenkins | 58.58 | The Bob English 2021 |
| 5 | PHAM, Nathan | JR | Robinson | 58.61 | Leto March 10th 2021 |
| 6 | KELLY, De'Qune | JR | Lakeland Christian | 59.03 | Lakeland City Meet 2021 |
| 7 | THOMAS, Jamar | SO | Lake Gibson | 1:00.50 | Lakeland City Meet 2021 |
| 8 | FLORENCE, Landon | 8 | Victory Christian (LL) | 1:02.52 | George Jenkins Tri Meet |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TAYLOR, Edino | SR | Winter Haven | 58.32 | The Bob English 2021 |
| 2 | LANGS, Robert | FR | Lake Wales | 58.18 | Lake Mary Invitational 2021 |
| 3 | HARRIS, Dwayne | SR | Haines City | 56.78 | The Ridge Championship 2021 |
| 4 | JONES, Jorde | SR | George Jenkins | 56.50 | |
| 5 | BURTON, Jeremiah | JR | Victory Christian (LL) | 56.68 | Calvary Christian Warrior Invite 2021 |
| 6 | DENTON, William | JR | Lake Wales | 58.03 | Lake Mary Invitational 2021 |
| 7 | ANNETT, John | SO | Newsome | 58.19 | Kowboy Invitational |
| 8 | COLE, Kamarion | FR | Auburndale | 58.34 | Kowboy Invitational |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CAMACHO, Joy | JR | Lake Wales | 56.07 | Lake Mary Invitational 2021 |
| 2 | DIFO, Xavier | JR | Robinson | 56.05 | Jesuit Quad Meet 2 |
| 3 | BROOKS, Tyrell | SO | Lakeland | 55.82 | Lakeland City Meet 2021 |
| 4 | TOLLIVER, Henry | SO | Haines City | 54.86 | George Jenkins Invite 2021 |
| 5 | POLLARD, Jerry | Auburndale | 54.93 | St. Cloud Track Invitational | |
| 6 | TILLERY, Ahmad | McKeel | 55.88 | Lakeland City Meet 2021 | |
| 7 | VELOSO, Joshua | McKeel | 56.06 | Lakeland City Meet 2021 | |
| 8 | CLEVELAND, Solomon | FR | Lakeland Christian | 56.29 | St. Cloud Track Invitational |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MYRICK, Terrance | SO | Lake Gibson | 54.61 | Lakeland City Meet 2021 |
| 2 | NELSON, Alijah | JR | Newsome | 54.13 | Kowboy Invitational |
| 3 | ARNONE, John | SR | Newsome | 52.94 | East Bay Tri-Meet |
| 4 | JOSHWAY, Caleb | Auburndale | 51.37 | FHSAA 3A District 8 and 4A District 5 | |
| 5 | BENDROSS, Gavin | JR | George Jenkins | 51.42 | Lakeland City Meet 2021 |
| 6 | COCHRAN, Tydarius | SR | Robinson | 53.22 | |
| 7 | FORSYETH, Keno | JR | Winter Haven | 54.40 | The Ridge Championship 2021 |
| 8 | LANG, Preston | JR | Lake Gibson | 54.61 | Lakeland City Meet 2021 |