| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Lake Brantley | A | Espinosa | Brooks | Montanez | Baker | 51.30 | FHSAA 4A District 2 |
| 4 | Seminole (Sanford) | A | Allen | Hillery | Soremi | Washington | 46.46 | 2018 FHSAA State Track & Field Championships |
| 5 | Lyman | A | Aaliah | Rentie | Ready | Gordon | 50.24 | FHSAA Class 4A-District 2 |
| 6 | Lake Mary | A | Gaspar | Bryant | Upshaw | Bullard | 56.50 |