| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bradenton Christian | A | Potocki | Velasquez | Cunard | Mcarthur | NT | |
| 2 | Cardinal Mooney | B | Maloni | Gregory | Ruben | Donovan | 13:23.04 | Sarasota Booker Relays |
| 3 | St. Stephens Episcopal | A | Fields | Jensen | Sket | Phommachanh | 12:36.61 | Sarasota Booker Relays |
| 4 | Cardinal Mooney | A | Sassano | Russo | Sutthoff | Hassler | 13:23.04 | Sarasota Booker Relays |
| 5 | Parrish | A | Limbaugh | Giles | Harmon | Strunk | NT | |
| 6 | Parrish | B | Seiler | VanBerkel | Cotton | Limon | DNS |