| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | GEORGES, REBECCA | Oak Ridge | NT | |
| 4 | LEGGIERO, Sofia | Hagerty | 15.70 | SAC Freshman Sophomore Championships 2021 |
| 5 | WILSON, Indira | University (Orlando) | NT | |
| 6 | LUGO PEREZ, Yariana | East River | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | SERRANO, Keilani | East River | 14.75 | Silverhawk Open 2021 |
| 2 | SILLS, Shyniyah | University (Orlando) | 14.44 | Metro East Championship 2021 |
| 3 | MANNING, LAKISHA | Oak Ridge | 14.02 | Cypress Creek (Orlando) Home Meet 1 |
| 4 | LOWE, Mikiya | Lake Howell | 13.74 | SAC Freshman Sophomore Championships 2021 |
| 5 | EGERTON, Kayla | Oviedo | 13.79 | Silverhawk Quint 2021 |
| 6 | LUNDSTROM, Megan | Hagerty | 14.21 | Silverhawk Quint 2021 |
| 7 | FLORES, Aurora | Geneva School | 14.60 | Silverhawk Open 2021 |
| 8 | LOWE, Serena | Lyman | 15.22 | SAC Freshman Sophomore Championships 2021 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BROWN , U'nitee | Winter Springs | 13.65 | SAC Freshman Sophomore Championships 2021 |
| 2 | CONNELLY, Kayla | Lake Howell | 13.50 | |
| 3 | SMITH, Kanya | Oviedo | 13.32 | FHSAA 4A District 2 |
| 4 | WASHINGTON, Jaylin | Seminole (Sanford) | 13.10 | Silverhawk Open 2021 |
| 5 | RENTIE, Ahnalea | Lyman | 13.29 | Silverhawk Open 2021 |
| 6 | HUNT, Kaelyn | University (Orlando) | 13.40 | Practice |
| 7 | SMITH, Karrngton | Winter Springs | 13.51 | 3rd Annual Windermere Meet of Champions Classic |
| 8 | READY, Sariah | Lyman | 13.71 | SAC Freshman-Sophmore 2020 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | LATIMER, Courtney | Oviedo | 13.04 | Silverhawk Quint 2021 |
| 2 | MCGRATH, Molly | Hagerty | 12.89 | Charlie Harris Relays 2021 |
| 3 | HUET, Rylee | Winter Springs | 12.69 | Silverhawk Open 2021 |
| 4 | WATSON, Makayla | Lake Howell | 12.50 | Lake Howell QUAD 2020 |
| 5 | RIDEOUT, Nicolette | East River | 12.52 | Metro East Championship 2021 |
| 6 | SOREMI, Oludoyin | Seminole (Sanford) | 12.79 | Lake Brantley Quad 2021 |
| 7 | SOREMI, Oluwadara | Seminole (Sanford) | 12.97 | Lake Brantley Quad 2021 |
| 8 | GEORGES, DEBRA | Oak Ridge | 13.10 |