| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LYNN, Remorreyan | JR | Gibbs | 2:20.00 | |
| 2 | BRADLEY, Jackson | SO | Seminole | 2:20.87 | Mustang Invitational |
| 3 | MAHONEY, Brendan | JR | Clearwater | 2:22.03 | 2:22.03 East Lake Early Bird Invitational |
| 4 | ANDRINGA, Brayden | FR | St. Petersburg | 2:22.12 | Warhawk Invitational |
| 5 | FASCE, Christian | JR | Boca Ciega | 2:26.15 | Mustang Invitational |
| 6 | HARRIS, Al'zaveon | SO | Gibbs | 2:29.30 | Warhawk Invitational |
| 7 | ROMAN, Jaiden | JR | Pinellas Park | 2:31.35 | Richard Allen Invitational |
| 8 | KELESKE, Timothy | JR | Clearwater | 2:32.04 | Calvary Christian Warrior Invite 2021 |
| 9 | MISHCHENKO, Alexander | SO | Clearwater | 2:33.81 | Calvary Christian Warrior Invite 2021 |
| 10 | WETHINGTON, d | SR | Boca Ciega | 2:45.00 | |
| 11 | PU, Joshua | FR | Largo | 2:48.20 | Fast Times at The Park |
| 12 | FIGARD, Kaleb | SO | Pinellas Park | 2:49.17 | Warhawk Invitational |
| 13 | LE, John | FR | Dixie Hollins | 2:55.67 | Fast Times at The Park |
| 14 | HARRIS, Isreal | SO | Gibbs | 2:57.59 | Fast Times at The Park |
| 15 | BETHUNE, Jayln | FR | Gibbs | NT | |
| 16 | FARRELL, Jayson | SR | Clearwater | NT | |
| 17 | MCGEE, Sincere | FR | Lakewood | NT | |
| 18 | MCNEALY, Jamal | SO | Lakewood | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CARTER, Randall | SR | Palmetto | 2:03.75 | North Port Invite 2021 |
| 2 | STONE, William | JR | Osceola (Seminole) | 2:04.45 | East Lake Early Bird Invitational |
| 3 | JENSEN, David | JR | Osceola (Seminole) | 2:05.88 | Warhawk Invitational |
| 4 | CHASE, Logan | FR | Seminole | 2:09.12 | Warhawk Invitational |
| 5 | GORDON, Javarious | SR | Largo | 2:09.96 | 2:09.96 57th Annual Ed Wells Jr.-Kiwanis Invitational |
| 6 | D'ANGIO, Wes | SO | Osceola (Seminole) | 2:10.00 | |
| 7 | KRIEGER, Kameron | SR | Boca Ciega | 2:10.00 | |
| 8 | YACOBI, Jonathan | SR | Largo | 2:10.01 | Richard Allen Invitational |
| 9 | MCMANUS, Ian | JR | Osceola (Seminole) | 2:12.00 | |
| 10 | CARLOS, Luis | SR | Palmetto | 2:12.51 | Palmetto Varsity Invitational |
| 11 | IRBY, Jay | JR | St. Petersburg | 2:13.16 | Warhawk Invitational |
| 12 | SUMMERS, Cole | SO | Lakewood | 2:14.00 | |
| 13 | STAVELEY, Alexander | SR | Largo | 2:14.05 | Ed Wells Jr.-Kiwanis Invitational 2021 |
| 14 | MCCLOUD, Malachi | SO | Boca Ciega | 2:15.00 | |
| 15 | OLSEN, Hunter | SR | Seminole | 2:15.00 | |
| 16 | WILSON, Kendrick | SO | St. Petersburg | 2:17.00 | |
| 17 | MEDARIES, Stevmoney | SR | Lakewood | 2:18.00 | |
| 18 | CATALDO, Shane | JR | Seminole | 2:18.33 | Richard Allen Invitational |