| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BRUCE, Sophia | SO | Boca Ciega | 2:48.00 | |
| 2 | MCINTOSH, Jordan | FR | Lakewood | 2:50.00 | |
| 3 | DENSON, Mae | FR | St. Petersburg | 2:52.87 | Mustang Invitational |
| 4 | COOPER, Kelsey | JR | Clearwater | 2:53.00 | |
| 5 | FORNSHELL, Lauren | JR | Clearwater | 2:53.33 | Calvary Christian Warrior Invite 2021 |
| 6 | NARCISSI, Amelia | SO | Seminole | 2:55.24 | Richard Allen Invitational |
| 7 | STAPLETON, Adriana | SO | Boca Ciega | 2:58.21 | 89th Annual West Coast Invitational |
| 8 | SHACKELFORD, Kori | JR | Clearwater | 2:59.10 | FHSAA 3A District 10 |
| 9 | PAYTON, Imari | FR | Lakewood | 3:00.99 | Richard Allen Invitational |
| 10 | EVANS, Kathryn | FR | Gibbs | 3:01.47 | St. Petersburg City Championship 2021 |
| 11 | HARRIS, Kara | FR | Clearwater | 3:03.00 | |
| 12 | WHEELINGS, Kenonie | FR | Lakewood | 3:04.88 | St. Petersburg City Championship 2021 |
| 13 | MARES, Ashley | JR | Pinellas Park | 3:05.49 | Warhawk Invitational |
| 14 | LAVINE, Gabrielle | SR | Gibbs | 3:20.78 | Spring Break Invite at IMG |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HILLMAN, Delaney | SR | Osceola (Seminole) | 2:20.00 | |
| 2 | REPKA, Katherine | JR | Seminole | 2:27.30 | 2:27.30 57th Annual Ed Wells Jr.-Kiwanis Invitational |
| 3 | NEWSOM, Mia | SO | St. Petersburg | 2:28.00 | |
| 4 | EAGAN, Ashley | JR | St. Petersburg | 2:30.00 | |
| 5 | TRIPP, Julia | SO | Osceola (Seminole) | 2:30.00 | |
| 6 | HENRIKSON, Ally | FR | Boca Ciega | 2:30.49 | Richard Allen Invitational |
| 7 | LATTO, Avery | FR | Osceola (Seminole) | 2:35.00 | |
| 8 | PINTO, Heloise | FR | Osceola (Seminole) | 2:35.00 | |
| 9 | POLINSKY, Emma | SR | Boca Ciega | 2:37.48 | Mustang Invitational |
| 10 | MADRID, Natalia | JR | Palmetto | 2:37.92 | Palmetto Varsity Invitational |
| 11 | RALPH, Abigail | SO | Seminole | 2:39.34 | Mustang Invitational |
| 12 | HAYWOOD, Angelina | JR | Seminole | 2:39.62 | Mustang Invitational |
| 13 | MATHENEY, Maya | FR | St. Petersburg | 2:42.30 | Mustang Invitational |
| 14 | YORK, Jillian | Largo | 2:45.54 | Seminole Warhawk Invitational | |
| 15 | HAMER, Jeanne | Largo | 2:45.81 | Warhawk Invitational |