| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | COLON, Stephanie | University (Orlando) | 18.87 | Spruce Creek George Janan Invitational |
| 2 | KISSENBERTH, Emily | Master's Academy (Oviedo) | 18.58 | FHSAA Class 1A-District 9 |
| 3 | ERIKSON, Ellie | Lake Mary | 18.03 | Brian Jaeger Elite Classic 2019 |
| 4 | SORENSEN, Eden | Lake Highland Prep | 15.70 | |
| 5 | ARCHIBALD-BOWERS, Giselle | Timber Creek | 16.61 | Jeff Wentworth Relays 2019 |
| 6 | MOORE, Payton | Winter Park | 18.24 | Trinity Prep Invitational 2019 |
| 7 | WHITE, Mallory | Winter Park | 18.80 | Metro Conference Freshman - Sophomore Championship 2019 |
| 8 | MARSTON, Marlia | Lake Nona | 18.90 | Tohopekaliga Tigers Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | DAVIS, Sarah | Master's Academy (Oviedo) | 20.78 | Lake Mary Wednesday Meet #1 2019 |
| 2 | MUKOIE-CARCAMO, Diana | Master's Academy (Oviedo) | 20.01 | Lake Mary Wednesday Meet #1 2019 |
| 3 | KUREYA, Valerie | Trinity Prep | 19.68 | Mount Dora Christian Invitational 2018 |
| 4 | YARED, Hannah | Trinity Prep | 19.40 | Saints Invitational 2019 |
| 5 | MARTINO, Chelsee | Lake Nona | 19.59 | Larry Kelly Invitational 2019 |
| 6 | AVERY, Caroline | Trinity Prep | 19.96 | Brian Jaeger Elite Classic 2019 |
| 7 | FLAY, Ashley | Lake Mary | 20.36 | SAC Freshman-Sophomore Championship 2019 |
| 8 | GAGLIARDI, Emily | Lake Mary | 21.15 | Brian Jaeger Elite Classic 2019 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | MARTINEZ, Amanda | Master's Academy (Oviedo) | 22.03 | Lake Mary Wednesday Meet #1 2019 |
| 4 | DAVID, Jennay | Lake Nona | 21.54 | Saint Cloud Invitational 2019 |
| 5 | PICHARDO, Arianna | East River | 21.74 | Metro Conference Freshman - Sophomore Championship 2019 |
| 6 | ESTRELLA, Kiara | Timber Creek | 22.08 | Highlander Classic 2019 |