| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lake Highland Prep | A | Miller, Samuel | Phillips, Jai | Walton, Joshua | Jacobs, Isaac | 8:08.71 | 2018 FHSAA State Track & Field Championships |
| 2 | Master's Academy (Oviedo) | A | Merly, Zach | Wilbanks, Josh | Rodriguez, Zhane | Daher, Michael | DNS | 8:33.41 Brian Jaeger Elite Classic 2019 |
| 3 | East River | A | Abbott, Jason | Morris, DeRel | Aring, Shawn | Aquino, Eric | 8:45.00 | |
| 4 | Boone | A | Cunningham, Cody | Hornbeck, Ethan | Phillips, Omarion | Correa, Brayan | 8:55.67 | |
| 5 | Cypress Creek | A | Jimenez-Abreu, Joshua | Torres Plaza, Miguel | Portillo, Erick | Urquhart, Rohan | 9:26.63 | |
| 6 | Lake Mary | A | Youngren, Nicholas | Whelton, Thomas | Gonzalez, Justin | Gonzalez, Brandon | 9:30.00 | |
| 7 | Geneva School | A | Shafer, Nick | Andreasen, Zachary | Miller, Joseph | White, Martin | 9:31.00 | |
| 8 | First Academy (Orlando) | A | Robinson, Jonathan | Rios, Esteban | Seppala, Cole | Albers, Brandon | 9:45.00 | |
| 9 | Winter Park | A | Lee, Parker | Zemliansky, Nikolai | Rhoads, Hector | Martorell, Mauricio | NT | |
| 10 | Lake Nona | A | Goyita, Victor | Scott, Nicholas | Fletcher, Owen | Sangmeister, Jack | NT | |
| 11 | Timber Creek | A | Cipion, Misael | Richardson, Marco | Al Hilali, Khalid | Varnedore, Lucas | NT |