| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Gayton, Samantha | SR | Father Lopez | 5:30.00 | |
| 2 | McDonnell, Claire | SR | Father Lopez | 5:30.00 | |
| 3 | Stansfield, Lyvia | South Lake | 5:55.00 | ||
| 4 | McCart, Jordan | SO | South Lake | 6:00.00 | |
| 5 | Taddeo, Ruth | FR | Belleview | 6:10.00 | |
| 6 | Mangandi Lazo, Paola | SO | Belleview | 6:20.00 | |
| 7 | Gordon, Aubree | JR | Belleview | 6:29.00 | |
| 8 | Lee, Alyssa | South Lake | 6:45.00 | ||
| 9 | Koshiol, Abigail | SR | DeLand | 6:45.65 | Jeff Wentworth Relays 2019 |
| 10 | Nunes, Emma | SO | Belleview | NT | |
| 11 | Annesi, Rachel | SO | Spruce Creek | NT | |
| 12 | Bronson, Madelyn | SR | Mainland | NT | |
| 13 | Bunn, Skyler | JR | University (OC) | NT | |
| 14 | Coleman, Catherine | JR | Seabreeze | NT | |
| 15 | Coleman, Kelly | FR | Seabreeze | NT | |
| 16 | Dane, Nickole | FR | Seabreeze | NT | |
| 17 | DiMuro, Cheyenne | FR | DeLand | NT | |
| 18 | Droker, Ilari | FR | Seabreeze | NT | |
| 19 | Ehlinger, Ashley | SR | University (OC) | NT | |
| 20 | Erickson, Jaylin | SO | Lake Weir | NT | |
| 21 | Evans, Estoria | SR | DeLand | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Fam, Karen | SR | Mainland | NT | |
| 2 | Frederico, Veronica | SR | DeLand | NT | |
| 3 | Galarza, Alana | SO | DeLand | NT | |
| 4 | Klingenberg, Ashley | SR | Lake Brantley | NT | |
| 5 | Lagos, Naobi | FR | DeLand | NT | |
| 6 | Lambert, Jayden | FR | Mainland | NT | |
| 7 | Lemus, Ashley | SO | Spruce Creek | NT | |
| 8 | Medina-Torres, Amanda | JR | Hagerty | NT | |
| 9 | Megge, Payton | JR | Spruce Creek | NT | |
| 10 | Miller, Emma | SR | Lake Brantley | NT | |
| 11 | Smith, Kendrie | SO | Belleview | 7:30.00 | |
| 12 | Peck, Cinnamon | SR | Lake Weir | NT | |
| 13 | Reynolds, Genevieve | SR | University (OC) | NT | |
| 14 | Rodd, Erin | SO | Hagerty | NT | |
| 15 | Sholar, Caroline | FR | University (OC) | NT | |
| 16 | Stites, Emma | FR | Trinity Christian (Deltona) | NT | |
| 17 | Thompson, Charissa | SO | Hagerty | NT | |
| 18 | Tighiovart, Yelena | FR | Lake Brantley | NT | |
| 19 | Usher, Abigail | JR | DeLand | NT | |
| 20 | Warren, Anna | FR | DeLand | NT | |
| 21 | Wheatley, Rachel | JR | Spruce Creek | NT | |
| 22 | Wilken-Yoder, Kathe | JR | Hagerty | NT | |
| 23 | Woods, Victoria | SO | Father Lopez | NT |